The VPS35 protein is a component of the retromer complex and participates in the recycling of membrane proteins via retrograde transport from endosomes back to the trans-Golgi network ( Bonifacino and Hurley, 2008 ).Is Vps35 downregulated in Alzheimer’s disease?
Western blotting confirmed the abnormality, establishing that VPS35 levels are reduced in brain regions selectively vulnerable to AD. VPS35 is the core molecule of the retromer trafficking complex and further analysis revealed that VPS26, another member of the complex, is also downregulated in AD.How does the Vps35-Vps29-Vps26 complex activate retrograde trafficking?
The association of the Vps35-Vps29-Vps26 complex to the cytosolic domains of cargo molecules endosomal membranes initiates the activation of retrograde trafficking and cargo capture. The nucleation complex was formed through the interaction of VPS complex with GTP -activated Rab7 with clathrin, clathrin-adaptors and various binding proteins.What is the isoelectric point of a Vps35 protein?
VPS35 was first identified in Saccharomyces cerevisiae from a study investigating the formation of lysosome-like vacuoles and sorting of vacuolar proteins. The protein contains 796 amino acid residues, with a molecular mass of 92 kDa and an isoelectric point of 5.32.